Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJM (1) Ciência Rural (1) Autor Camargos,M. F. (1) Camargos,Marcelo Fernandes (1) D´Ambros,Régia Maria (1) Fonseca Jr.,A. A. (1) Fonseca Junior,Antônio Augusto (1) Guedes,Estefânia Oliveira (1) Heinemann,M. B. (1) Heinemann,Marcos Bryan (1) Leite,R.C. (1) Lima,Graciela Kunrath (1) Nonaka,Carolina Kymie Vasques (1) Reis,J. K. P. (1) Sales,M. L. (1) Mais... Palavra-chave Pseudorabies (2) Bioinformatics (1) Diagnose (1) Glycoprotein (1) Real time PCR (1) Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2012 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Different methods of real-time PCR for detection of pseudorabies virus Ciência Rural Nonaka,Carolina Kymie Vasques; Fonseca Junior,Antônio Augusto; Guedes,Estefânia Oliveira; D´Ambros,Régia Maria; Lima,Graciela Kunrath; Camargos,Marcelo Fernandes; Heinemann,Marcos Bryan. ABSTRACT: Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine industry. The aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the molecular diagnosis of PR. Eight primer pairs targeting four genes (gB, gC, gE, gD), three different qPCR chemistries (SybrGreen, hydrolysis probes and plexor) and two equipment (ABI7500, Rotorgene 3000) were evaluated. Oligonucleotides targeting gB using hydrolysis probes showed the best performance after evaluating efficiency (99%), the detection limit (10-1.5 TCID50 mL-1) and diagnostic sensitivity and; therefore, those primers were selected for performance verification factors such as repeatability,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Real time PCR; Pseudorabies; Diagnose. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300452 Pseudorabies virus can be classified into five genotypes using partial sequences of UL44 BJM Fonseca Jr.,A. A.; Camargos,M. F.; Sales,M. L.; Heinemann,M. B.; Leite,R.C.; Reis,J. K. P.. Suid herpesvirus 1 (SuHV-1) is the causative agent of pseudorabies (PR), a disease of great importance due to the huge losses it causes in the swine industry. The aim of this study was to determine a method for genotyping SuHV-1 based on partial sequences of the gene coding for glycoprotein C (gC) and to elucidate the possible reasons for the variability of this region. A total of 109 gCsequences collected from GenBank were divided into five major groups after reconstruction of a phylogenetic tree by Bayesian inference. The analysis showed that a portion of gC (approximately 671 bp) is under selective pressure at various points that coincide with regions of protein disorder. It was also possible to divide SuHV-1 into five genotypes that evolved under... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudorabies; Glycoprotein; Bioinformatics. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400048 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco